In the world of diagnostics, the Enzyme-Linked Immunosorbent Assay (ELISA) has long been a cornerstone technique for detecting and quantifying proteins, antibodies, and hormones. It is widely used across clinical laboratories, pharmaceutical research, and various fields in biomedical science. While the technique itself is robust and reliable, the interpretation of ELISA results can sometimes be challenging due to factors like variability in reagent quality, subtle procedural differences, and
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